Catalog Number - 68304-10P-3M

Cogent RP C4 Butyl™ Wide Pore is a reversed-phase HPLC stationary phase specifically designed for the separation of proteins, peptides, enzymes, and other large biomolecules. Manufactured using a traditional butyl (C4) bonded phase on ultra-high purity silica and fully double end capped, this 300 Å wide pore chemistry provides enhanced accessibility for larger molecules compared to standard 100 Å pore columns. The wide pore structure improves mass transfer and interaction with the stationary phase, making it an excellent choice for bioanalytical, biopharmaceutical, and biomolecule characterization applications.

68304-10P-3M is an HPLC column packed with 3 µm RP C4 Butyl™ Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 100 mm format. The 100 mm length provides increased resolution and peak capacity compared to shorter columns, while the 3 µm particles deliver greater efficiency and sharper peaks than comparable 5 µm packings. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and instrument configuration.

This column is particularly well suited for proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules analyzed by reversed-phase HPLC. The C4 chemistry provides lower hydrophobic retention than C8 phases, which is often advantageous for biomolecules that may be excessively retained on more hydrophobic stationary phases. The 300 Å pore structure allows large molecules to fully access the bonded phase, resulting in improved recovery and separation performance.

Key Benefits

• RP C4 Butyl™ Wide Pore stationary phase

• Fully double end capped

• Traditional butyl (C4) bonded phase

• Ultra-high purity silica support

• 300 Å wide pore design

• Optimized for proteins and peptides

• 3 µm particle technology

• Greater efficiency than 5 µm columns

• Enhanced chromatographic resolution

• Excellent for bioanalytical methods

Typical Applications

• Protein analysis

• Peptide analysis

• Enzyme characterization

• Bioanalytical methods

• Biopharmaceutical analysis

• Protein purity testing

• Peptide mapping

• Biomolecule separations

• Method development

• Reversed-phase HPLC

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C4 Butyl™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 300 Å

• Column Dimensions: 4.6 mm ID x 100 mm

• Length: 100 mm

• Typical Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• Designed for proteins, peptides, enzymes, and other large biomolecules

• 300 Å wide pores improve access to the bonded phase for larger molecules

• Suitable for conventional HPLC systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 100 mm length provides greater resolution and peak capacity than 50 mm columns

• Lower retention than comparable C8 stationary phases

• Excellent choice for biopharmaceutical and biotechnology laboratories

• Particularly useful for large hydrophobic and neutral biomolecules

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